This second training school, jointly organized by MEDIS (Université Clermont Auvergne, France) and CMET (Ghent University, Belgium), aims to train about complex dynamic in vitro gut models, with a particular focus on systems integrating oral and small intestine microbiota, high-throughput models and diseased-related conditions. This training school is directly linked to the goal of WG2 “extension to other gastrointestinal compartments” and WG3 “extension to diseases conditions”. Training will be performed on a large range on in vitro models including TIM, ARCOL, ARILE, ESIN, SHIME, mini-bioreactors and anaerobiosis methods.