Working Group 1

Review of existing in vitro human colon models and harmonized guidelines

Objectives and Activities

Review of existing in vitro human colon models and establishment of harmonized guidelines to enable better comparison of data from different models. This WG is dedicated to the development of harmonized procedures and in vitro gut models. So far, we have produced 6 WG meetings and a first draft of the first part of Deliverable 1.1, that is now under review. At the 1st INFOGUT annual Meeting WG1 presented more than a dozen posters and three oral communications. We have created several shared folders on Google Drive plenty of documents and presentations. We have produced a survey table that was fulfilled by WG1 applicants that represents the 1st Milestone of the whole INFOGUT project. We are preparing also a glossary of definitions. WG1 has organized the 1st INFOGUT Annual Meeting and is producing the Proceedings of the 1st INFOGUT Annual Meeting. WG1 has generated STSM exchanges.

Working group leaders

Lorenzo Nissen

WG1 Leader
Alma Mater Studiorum – University of Bologna

Harsh Mathur

WG 1 Co-Leader
Teagasc – Agriculture And Food Development Authority

Tasks

Task 1.1. Harmonization of fermentation protocols (batch, continuous, multi-assays)

Review and map of the current in vitro model systems with the objective of identifying key parameters that require standardization across all models.

Task 1.2. Inoculum (faeces, mock communities), fermentation medium and control

Apply the standardized parameters across the range of in vitro colonic models available within the INFOGUT network to establish the consistency and repeatability of gut microbiota readouts under standardized conditions.

Task 1.3. Non-human gut models

Based on the same approach adopted in Tasks 1.1 and 1.2, review the available protocols and experimental conditions to propose standardized parameters for in vitro animal gut models

Task 1.4.

Prepare a peer review publication on the agreed standardized parameters and their application across the available in vitro colonic models, highlighting the key benefit of their application.

Deliverables

D1.1

Report mapping the current in vitro colon models, their key process parameters, standardizations and quality control procedures across the range of in vitro colonic models available within the COST network. Due time at month 15

D1.2 Non-human gut models

Review and map of currently in vitro model systems and identification of standardized parameters. Due time at month 24

D1.3

Submitted manuscript on the agreed standardized parameters and their application across the available in vitro colonic models. Due time at month 42

Working group meetings

COST

COST (European Cooperation in Science and Technology) is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. (www.cost.eu)

Cost EU
COST Action CA23110

Scientific literature is shedding light on the centrality of GI for human health and wellbeing. Indeed, the physiologic effects of nutrients, bioactives and even toxic compounds (including foodborne pathogens) are mediated by their absorption rate in the intestine and by their interaction with gut microbiota and its host ecosystem. Testing food, feed, supplements or drugs in clinical studies gives rise to ethical issues, and the transferability of animal data across species is often problematic because of differences in physiology, metabolism and chemical susceptibilities.

Discover

MoU
062/22

CSO Approval Date
27/05/2022

Start Date
06/10/2022

End Date
05/10/2026

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